Name: China Original manufacturer Hot Selling High Quality Polysucrose 400 CAS 26873-85-8 with good price
Brand: Wuhan Fortuna Chemical Co., Ltd.

Description of Polysucrose from Wuhan Fortuna Chemical Co.,Ltd

Basic Information of Polysucrose
Product Name	Polysucrose
CAS NO.	26873-85-8
Apperance	White,odourless powder
Assay	99.50%
Pacakge	1kg/Foil Bag,25kg/Drum/Box
MOQ	1kg
Storage	Cool and dry place
Shelf Life	2 Years
Brand	Fortunachem

Specifications(COA) of High Quality Research Reagent Ficoll400 Polysucrose CAS NO 26873-85-8 In Stock

Items	Specifications	Results
Description	White,odourless powder	Conform
Identity	Positive	Conform
Specific optical rotation(a)D20	53-59	55
Intrinsic viscosity (20℃）	14-20	16
Molecular weight(%)	Ca.400000	400000
Loss on drying(%)	≤ 2%	1.5%
Assay(%)	99%～100%	99.50%
PH- value(10%w/v aqueous solution)	7.0～8.50	7.0
Sulphated ash(%)	≤0.3%	0.1%
Content of chloride (ppm)	≤500 ppm	55
Sterilization test	Corresponding	Corresponding
Conclusion	The product conforms to the standard of CP2010.

Polysucrose 400 is a synthetic, highly branched polysaccharide polymer widely used in biochemistry and cell biology, primarily as a key component in density gradient media for cell separation. Its chemical and physical properties make it exceptionally well-suited for this purpose.

Chemical Composition and Synthesis
It is produced by the copolymerization of sucrose (a disaccharide) with epichlorohydrin. This reaction creates a three-dimensional, cross-linked network. The "400" in its name denotes its average molecular weight, which is approximately 400,000 Daltons. The structure is densely populated with hydroxyl (-OH) groups from the sucrose monomers, making the molecule highly hydrophilic.

Key Properties

High Hydrophilicity and Solubility: The abundance of hydroxyl groups allows Polysucrose 400 to dissolve readily in water and form stable, viscous solutions.
Biologically Inert: It is non-toxic and does not interact with or activate most biological systems. Cells remain viable when suspended in its solutions.
Non-Ionic: Unlike ionic compounds like cesium chloride, Polysucrose 400 is electrically neutral. This is critical because it prevents osmotic shock to cells; it does not dissociate into ions that could create an unfavorable osmotic environment.
High Molecular Weight and Non-Penetrability: Due to its large, branched structure, it cannot cross intact cell membranes. This ensures that the density of the medium outside the cell is what determines its buoyancy, not the internal composition of the cell.
Low Osmolality: Despite its high molecular weight, it can be used to create high-density solutions with relatively low viscosity and, most importantly, low osmolality. This is a significant advantage over smaller molecules like sucrose, which require high molar concentrations to achieve the same density, resulting in hyperosmotic solutions that can damage or shrink cells.

Primary Application: Density Gradient Centrifugation

The most prominent use of Polysucrose 400 is in the formulation of media like Ficoll-Paque™. In this application:

Principle: A solution of Polysucrose 400 is prepared at a specific density (e.g., 1.077 g/mL). When a blood sample is layered on top and centrifuged, cells separate based on their buoyant density.
Result: Mononuclear cells (lymphocytes, monocytes) form a distinct band at the sample-medium interface, while denser red blood cells and granulocytes pellet at the bottom.
Advantage: The combination of high density, low viscosity, and low osmolality provided by Polysucrose 400 allows for the gentle and efficient separation of viable, functional cells.

In summary, Polysucrose 400 is a chemically engineered polymer whose unique inert, non-ionic, and high-density characteristics make it an indispensable tool for the safe and effective separation of biological cells by centrifugation.

FAQ From Wuhan Fortuna Chemical Co.,Ltd
Q	Can I get a sample for free ?
A	For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test.

Q	How to confirm the product quality before placing orders ?
A	Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order.

Q	What is the payment you accept?
A	For small and low value order, we accept CreditCard, MaterCard,VISA,Western Union and T/T.For large order, we can do T/T and L/C. Any pay on delivery or pay-after are not available for us.

Q	What is your shipment way ?
A	For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement.

Q	How long is your after-sales service?
A	We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company.

Q	How do you treat quality complaint ?
A	We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers.

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide Polysucrose with good quality and best price, welcome to contact us to get COA/TDS/MSDS of Polysucrose.