Application of Carboxypeptidase B in Food

Name: Carboxypeptidase B (EC 3.4.17.2, CAS 9025‑24‑5)
Brand: Wuhan Fortuna Chemical Co., Ltd.

Carboxypeptidase B (EC 3.4.17.2) is a zinc‑dependent exopeptidase that selectively cleaves C‑terminal basic amino acids including arginine and lysine from peptide chainsPMC. As a food‑grade processing aid, it cooperates with endoproteases to achieve controlled protein hydrolysis and improve product quality across multiple food sectors, working best under pH 7.5‑9.0 in liquid systems.

Its primary food application lies in manufacturing protein hydrolysates from whey, soybean, casein and plant‑based raw materials. After endoprotease digestion, many short peptides bring unpleasant bitter notes. Carboxypeptidase B releases terminal basic residues, modifies peptide composition and reduces bitterness, making hydrolysates acceptable for human consumption. It is widely adopted for producing high‑F‑ratio oligopeptides, nutritious peptide ingredients used in functional foods, sports nutrition and special‑medical‑purpose food such as tube‑feeding formulas and infant supplementary food. These peptide products gain better digestibility because small fragments and free amino acids are easier for human intestines to absorb.

This enzyme also contributes to flavour enhancement in food processing. By liberating free basic amino acids, it enriches umami‑related flavour profiles in protein hydrolysates, meat extracts and yeast‑based seasonings. Instead of chemical degradation, this mild enzymatic method retains natural flavour characteristics without harsh by‑products.

In commercial production, porcine‑pancreatic‑sourced or recombinant carboxypeptidase B is dosed at low dosage during hydrolysis. Thermal inactivation follows enzymatic reaction, so no active enzyme remains in final food goodsPMC. It must comply with local food‑enzyme safety regulations. Compared with common food proteases, carboxypeptidase B remains relatively costly, so it is mostly applied in high‑value functional food ingredients rather than mass‑produced conventional food. It rarely appears as a final‑product ingredient and functions only as a processing auxiliary agent throughout food workflows.

Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.