Specifications of Subtilisin CAS 9014-01-1 COA:

Items	Specifications	Results
Appearance	Yellow brown powder	Yellow brown powder
Activity u/g	200,000u/g	214,331u/g
Moisture %	≤8.0	5.5
Conclusion	The product conforms to the above specifications.

Product Name:Subtilisin

CAS:9014-01-1

MF:N/A
Melting point :N/A
form:powder
color:Yellow brown
Uses:

Bacillopeptidase B (CAS 9014-01-1)

Bacillopeptidase B (CAS 9014-01-1), more commonly known as Subtilisin, is an extracellular alkaline serine protease secreted by Bacillus species, primarily Bacillus subtilis and Bacillus licheniformis. It belongs to the subtilase family (serine protease S8 family) and catalyzes the hydrolysis of peptide bonds in proteins through nucleophilic attack by an active-site serine residue.

Key Identity Information

Property	Detail
CAS Number	9014-01-1
EC Number	3.4.21.14
EINECS	232-752-2
Other Names	Subtilisin, Alkaline Protease, Protease A.S 1398, 枯草溶菌素, Subtilisin Carlsberg/BPN'
Molecular Weight	~27 kDa (Subtilisin A); range 20,000–45,000 Da depending on variant
Enzyme Class	Serine endopeptidase (alkaline protease)
Source Organisms	Bacillus subtilis, B. licheniformis, B. amyloliquefaciens
Optimum pH	8.0–12.0 (alkaline)
Optimum Temperature	50–60 °C (some variants up to 80 °C)

Structural & Biochemical Characteristics

Non-glycosylated single polypeptide chain of ~270 amino acid residues (some variants ~309 residues).

No disulfide bonds — a distinctive feature contributing to its stability in harsh alkaline environments.

Active site contains a catalytic triad (Ser-His-Asp) typical of serine proteases.

Stable across a wide pH range and retains significant activity in the presence of surfactants, oxidizing agents, and organic solvents.

Inhibited by DFP (diisopropyl fluorophosphate) and PMSF, confirming its serine protease mechanism.

Applications & Selling Points

Bacillopeptidase B / Subtilisin is one of the most commercially significant industrial enzymes in the world. Alkaline proteases account for approximately 35% of total global microbial enzyme sales, and subtilisin-type enzymes dominate the detergent sector.

1. Detergent Industry (Largest Market)

This is the single largest application, accounting for the majority of subtilisin consumption worldwide.

Stain removal: Efficiently hydrolyzes protein-based stains (blood, egg, grass, food) at alkaline pH (8–12) and moderate temperatures (30–60 °C).

Cold-active variants have been engineered for energy-saving low-temperature washing (15–30 °C), reducing energy consumption and preserving fabric texture.

Compatibility: Stable in the presence of surfactants, bleaching agents, and builders commonly found in laundry formulations.

Over 90% of enzyme-containing detergents in international markets contain subtilisin-type proteases.

Selling point: The global shift toward cold-water washing and eco-friendly detergent formulations is driving demand for engineered cold-active subtilisins — a high-growth segment.

2. Leather & Tanning Industry

Dehairing: Replaces traditional sodium sulfide/soda lime chemical processes. Subtilisin digests hair keratin at the root without damaging the collagen structure or opening pores, producing higher-quality leather.

Bating: Removes unwanted interfibrillary proteins after dehairing, producing softer, stretchier, silkier leather with improved tensile and tear strength.

Environmental advantage: Eliminates 100% of sulfide and 80% of suspended solids from tannery effluent waste — a major pollution reduction.

Selling point: Growing environmental regulations worldwide are pushing tanneries away from chemical processing toward enzymatic alternatives, creating expanding demand.

3. Silk Degumming & Textile Processing

Selectively removes sericin (the gummy outer coating) from silk fibers while preserving fibroin (the structural core), improving silk luster, softness, and dyeability.

Offers better control and gentler processing compared to traditional alkaline boiling methods.

Selling point: Premium textile markets (especially in China, India, and Southeast Asia) increasingly favor enzymatic degumming for superior silk quality.

4. Food & Flavor Industry

Protein hydrolysis: Accelerates breakdown of meat, fish, and plant proteins, shortening processing cycles.

Flavor enhancement: In soy sauce, fermented pastes, and condiment production, subtilisin promotes generation of flavor-active amino acids and peptides, optimizing taste profiles.

Bitterness reduction: Hydrolyzes bitter-tasting C-terminal hydrophobic amino acids from protein hydrolysates.

Bioactive peptide production: Used in combination with endoproteases to produce antioxidant and functional peptides for nutritional supplements.

In 2024, the European Food Safety Authority (EFSA) began developing usage regulations for subtilisin as a food additive, and in May 2026, EFSA assessed subtilisin produced by non-GMO B. licheniformis as safe under intended use conditions.

Selling point: The clean-label and natural food movement favors enzymatic processing over chemical hydrolysis, opening new market channels.

5. Animal Feed Industry

Added to poultry and livestock feed to improve protein digestibility, enhancing feed conversion ratios and promoting weight gain.

Studies show that subtilisin-supplemented broiler feed promoted 15% weight gain over controls.

Multi-enzyme formulations combining subtilisin with amylase, cellulase, and lipase show synergistic effects, reducing feed costs by 18–22% per kg of weight gain.

Selling point: With global meat consumption rising, feed efficiency is a critical cost driver — subtilisin-based feed enzymes offer measurable ROI for livestock producers.

6. Pharmaceutical & Biotechnology

Peptide synthesis: Can catalyze peptide bond formation in organic solvents (reverse proteolysis), useful for synthesizing small peptides and modified amino acid derivatives.

Protein engineering tool: Widely used in directed evolution studies due to its well-characterized structure and tolerance to mutation.

Anti-inflammatory research: Studies on bacillopeptidase from B. subtilis CFR5 showed significant reduction of acute pancreatitis severity in animal models, suppressing pro-inflammatory cytokines (IL-6, TNF-α, IL-1β) and restoring intestinal barrier integrity.

Fibrinolytic activity: Certain subtilisin variants exhibit clot-dissolving properties, being explored as thrombolytic agents.

Selling point: Emerging therapeutic applications in anti-inflammatory and thrombolytic areas represent high-value, high-margin opportunities.

7. Waste Treatment & Environmental Biotechnology

Degrades protein-rich industrial waste (slaughterhouse effluent, food processing wastewater).

Used in bioremediation of organic pollutants.

Optimal activity at pH 8.0 makes it suitable for neutral-to-alkaline waste streams without pH adjustment.

Selling point: Increasingly stringent environmental discharge regulations create steady demand for industrial biocatalysts in wastewater treatment.

8. Poultry & Feather Processing

Hydrolyzes feather keratin for conversion into digestible feather meal — a high-protein animal feed ingredient.

Converts what would otherwise be waste into a valuable nutritional product.

Summary of Key Selling Points

Selling Point	Description
Dominant market position	Accounts for ~35% of global microbial enzyme sales; the #1 industrial protease
Broad pH & temperature stability	Active at pH 8–12 and up to 60–80 °C — ideal for harsh industrial conditions
Detergent industry backbone	Essential ingredient in >90% of enzyme-containing detergents worldwide
Environmental compliance	Replaces polluting chemicals in tanning (eliminates sulfide waste) and enables eco-friendly processing
Cold-active engineering potential	Psychrophilic variants enable energy-saving low-temperature washing — a growing market
Food-grade safety	EFSA safety assessment completed (2026); GRAS status in the US; expanding food applications
Feed efficiency ROI	Measurable 15–22% improvement in feed conversion — direct cost savings for livestock producers
Versatile biocatalyst	Functions in both aqueous and organic solvents, enabling peptide synthesis and specialty chemistry
High-yield microbial production	Bacillus hosts secrete 20–25 g/L of extracellular enzyme — among the highest yields of any industrial enzyme
Emerging therapeutic potential	Anti-inflammatory and fibrinolytic activities open high-value pharmaceutical markets

In summary, Bacillopeptidase B (Subtilisin, CAS 9014-01-1) is a foundational industrial enzyme with an established multi-billion-dollar market presence, particularly in detergents, and is simultaneously expanding into high-growth segments including cold-active formulations, green leather processing, functional foods, animal feed, and biopharmaceuticals. Its combination of robust stability, high catalytic efficiency, microbial producibility at scale, and versatile substrate range makes it one of the most commercially successful enzymes in biotechnology history.

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FAQ From Wuhan Fortuna Chemical Co.,Ltd

Q1: Can I get a sample for free ?
A: For small QTY and low value product, we can provide sample for free exclude shipping cost. For high value sample, we will charge the basic sample cost, but we can refund all the cost if customers place order after sample test.
Q2: How to confirm the product quality before placing orders ?
A: Most of our products confirm to international standards like USP/EP/BP/JP/CP. We will provide COA/TDS/MSDS for customers to confirm the specification before placing order.
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A: For less than 100kg gross weight order will ship with courier like FedEx or UPS, we support customers to place courier orders remotely by their account, we will package the goods well and wait for pick up.For more than 100kg goods, we will transport by Air or Sea according to customers' requirement.
Q5: How long is your after-sales service?
A: We accept customer’s quality complaint in three month after customer received the goods. We provide tech.support for all-life if customer place order from our company.

Q6: How do you treat quality complaint ?

A: We support customer to provide our products to any rightful third party testing organization to test our products according to the COA specification, if the test result is confirm with COA, we will take all the loss for customers.

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Name:Wuhan Fortuna Chemical Co.,Ltd

Category:Others

Trade Category: Trading Company

Employees: 11 - 50 People

Address: Room 2015, No.2 Building, Kaixin Mansion No.107

Company Information: Wuhan Fortuna Chemical Co.,Ltd established in 2006, is a big integrative chemical enterprise being engaged in Pharmaceutical and Intermediates, Food and Feed additives, Fine chemicals,Biochemical and Reagent, which is the members of Hubei E-commerce Chamber.

Our company has professional persons who major in chemical R&D and sicentific management, supply fine chemical products with high quality and close service, also supply custom-tailored products according to our clients' requirement.

We have a positive and self-motivated management work team with a common philosophy, caring and commitment through teamwork, our team strive to achieve success in delighting our clients and ourselves.

Since Wuhan Fortuna Chemical Co., Ltd founded, we continuously innovate our products and improve our service, sales network. Hence, we initiate the first sale mode on net in China, which is the retail trade of small package bring along wholesale of diversified management modes. Our products are exported widely to South Korea, Vietnam, Australia, Europe and South America, highly recommended by our clients.We insist on the management creed “Market is our compass, Quality is our life, Credit is our soul”. Clients’ trust is our forward power, their satisfaction is our struggling goal.

Wuhan Fortuna Chemical Co.,Ltd is a professional manufacturer which provide Subtilisin with good quality and best price, welcome to contact us to get COA/TDS/MSDS of Subtilisin.

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