English 
Español 
français 
العربية Search
Search
FAST BLUE BB SALT is a laboratory chemical, specifically a diazonium salt, used as a staining agent in biology and medicine. Its primary use is in histology to detect the presence and location of specific enzymes, like alkaline phosphatase, in tissue samples.
It works by reacting chemically with enzyme byproducts to form an insoluble, bright blue/purple dye precisely where the enzyme is active. This allows scientists and pathologists to visualize cellular processes under a microscope, aiding in research and disease diagnosis. It is not a drug but a vital reagent, handled with care due to its light-sensitive and potentially hazardous nature.
Items | Specifications | Results |
Appearance | Yellow to green to brown powder | Conform |
Assay | 35% | Conform |
Solubility | 1mg/mL,hot H2O | Conform |
Conclusion | The product conforms to the above specifications. | |
FAST BLUE BB SALT is a chemical compound used primarily as a dye precursor and a coupling agent in histology, cytology, and biochemical staining. It is not a therapeutic drug like Rifampicin, but a laboratory reagent.
Here’s a detailed breakdown:
Chemical Name: Variously listed as Fast Blue BB, Fast Blue B Salt, or tetrazotized *o*-dianisidine. Its systematic name is Bis(diazonium) salt of o-dianisidine.
Type: It is a diazonium salt. Diazonium salts are highly reactive compounds that readily form covalent bonds with specific other chemicals (like phenols and aromatic amines) in a reaction called azo coupling.
Appearance: Typically supplied as a beige to brownish powder. It is light-sensitive and often requires refrigeration for storage.
The core function of Fast Blue BB Salt is to act as a coupling agent to produce an insoluble, colored precipitate (an azo dye) in situ (on the spot, where the reaction occurs). This is crucial for visualization.
1. Histology & Enzyme Histochemistry (Main Use)
This is its most significant application. It is used to localize the activity of specific enzymes in tissue sections (e.g., from biopsies).
Target Enzyme: Alkaline Phosphatase (ALP) and other hydrolytic enzymes.
Process (Example - Gomori Method for Alkaline Phosphatase):
A tissue section is incubated with a substrate molecule (commonly Naphthol AS-MX phosphate).
Where the enzyme Alkaline Phosphatase is present, it cleaves the phosphate group from the substrate, releasing the reactive intermediate (Naphthol AS-MX).
Fast Blue BB Salt (the diazonium salt) in the solution immediately couples with the liberated naphthol.
This coupling reaction forms an insoluble, brightly colored (blue/blue-purple) azo dye precipitate at the exact site of enzyme activity.
This allows a researcher or pathologist to see, under a microscope, precisely where and how active that enzyme is in the tissue (e.g., in bone-forming cells, liver bile ducts, or certain tumors).
2. Chromogenic Substrate for Detection Systems
It can be used in blotting techniques (e.g., Western, Southern, Northern) as part of a detection system when conjugated to other molecules, though other dyes are more common for this now.
3. Dye Manufacturing
As a diazonium salt, it is a fundamental component in the industrial synthesis of various azo dyes for textiles, inks, and pigments.
Light and Temperature Sensitive: It degrades over time, especially when warm or exposed to light. Must be stored cold, dark, and dry.
Hazardous: As a diazonium salt and an aromatic amine derivative, it is considered a potential irritant and health hazard. Safety Data Sheets (SDS) classify it as harmful if inhaled, in contact with skin, or swallowed. It may also be a potential mutagen.
Laboratory Use Only: It is strictly a research and diagnostic reagent, not for human or veterinary therapeutic use.
| Feature | Rifampicin | Fast Blue BB Salt |
|---|---|---|
| Primary Nature | Pharmaceutical Antibiotic | Chemical Reagent / Dye Precursor |
| Core Use | Treat infections (TB, leprosy) in humans/animals | Visualize enzymes in tissues/cells in a lab |
| Mechanism | Inhibits bacterial RNA polymerase | Couples with naphthols to form an insoluble colored dye |
| End Result | Kills bacteria, cures disease | Produces a blue stain for microscopic analysis |
| User | Patients, clinicians | Researchers, histotechnologists, pathologists |
FAST BLUE BB SALT is a diazonium salt used as a color-forming coupling agent in histology. Its main purpose is to visually localize enzyme activity (especially Alkaline Phosphatase) in tissue samples by producing a stable, insoluble blue precipitate at the site of enzyme activity. It is an essential tool for diagnostic pathology and biological research.
Fortunachem Provides Not Only Professional Chemical Products But Also Professional Help
Keeping you up-to-date with all the latest information, news, and events about Fortunachem!
Quick Links
Add:
E-mail:
