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CAPSO is a biological buffer agent used to maintain a stable pH in biochemical and molecular biology experiments. Its full chemical name is 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid.
Key properties:
Effective pH Range: 8.9 - 10.3 (most effective near its pKa of 9.6).
Common Use: It is particularly useful for enzymatic assays and procedures that require a stable alkaline environment, such as electrophoresis.
Key Feature: It is a zwitterionic (contains both positive and negative charges), "Good's buffer" known for being inert, highly soluble, and having minimal interference with biological processes.
| Item | Specification |
| Appearance | White powder |
| Assay | 99%min |
| Density | 1.29±0.1 g/cm3(Predicted) |
| Melting point | 270-274°C |
| Product parameters | |
| Cas number: | 73463-39-5 |
| Appearance: | White Powder |
| Purity: | 99% min |
| MOQ: | 1kg |
| Price: | Negotiable |
| Package details: | 1kg/foil bag |
| Brand: | Fortunachem |
Its fundamental purpose is to maintain a stable pH in biochemical and biological experiments. Fluctuations in pH can denature proteins, inhibit enzyme activity, disrupt cell membranes, and ruin sensitive experiments. CAPSO, like other Good's buffers, is specifically designed to prevent this.
Effective pH Range: Its most useful buffering range is between pH 8.9 and 10.3. It is most effective at its pKa value of 9.6 at 20°C (the point where it has maximum buffering capacity). This makes it ideal for procedures requiring a stable alkaline environment.
Zwitterionic Nature: CAPSO is a zwitterion, meaning it possesses both a positive and a negative charge on the same molecule. This property contributes to its:
High Water Solubility
Low Membrane Permeability (it doesn't easily cross cell membranes, making it suitable for cell culture work)
Minimal Reactivity and interference with biological systems
Other Advantages:
Chelation: It has minimal tendency to form complexes with metal ions (chelation), which is crucial for enzymatic reactions that require metal cofactors (e.g., Mg²⁺ or Ca²⁺).
UV Transparency: It does not absorb light significantly in the ultraviolet range, making it perfect for spectrophotometric assays and protein purification monitoring.
CAPSO is a staple reagent in many laboratory settings:
Biochemistry & Molecular Biology:
Enzyme Kinetics: Buffering enzymatic reactions that have optimal activity in the pH 9-10 range.
Gel Electrophoresis: Used in the buffer systems for certain types of protein and DNA separation techniques.
Chromatography: As a component of mobile phases in liquid chromatography (HPLC, FPLC) to separate biomolecules.
Cell Biology:
Cell Culture: Maintaining pH in specialized media for certain cell types or experimental conditions.
Organelle Isolation: Buffering solutions used to isolate cellular components like mitochondria or chloroplasts, which can be sensitive to pH changes.
Pharmaceutical Research: Used in drug discovery and development processes where pH control is critical for analyzing drug interactions and stability.
In summary, CAPSO is a specialized, zwitterionic buffer essential for maintaining alkaline pH stability in a wide array of biochemical, molecular biological, and cell-based experiments, where its inertness and specific properties prevent interference with delicate biological processes.
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