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DL-Dithiothreitol (DTT) is a strong reducing agent essential in biochemistry and molecular biology.
Key Points:
Function: Cleaves disulfide bonds (-S-S-) in proteins/peptides into sulfhydryl groups (-SH), disrupting protein structure and preventing unwanted oxidation.
Structure: Contains two thiol groups (HS–CH₂–CH(OH)–CH(OH)–CH₂–SH); "DL" denotes a racemic mixture of stereoisomers.
Major Uses:
Denaturing proteins for techniques like SDS-PAGE.
Protecting enzymes/nucleic acids from oxidation during experiments.
Maintaining reducing conditions in buffers.
Properties: Water-soluble, air-sensitive (oxidizes quickly), with a sulfurous odor.
Handling: Prepare solutions fresh or store frozen/under inert gas; use gloves/fume hood (irritant).
In short: DTT is indispensable for manipulating disulfide bonds and ensuring redox stability in biological research.
| Item | Specification |
| Appearance | White Powder |
| Assay | 99%min |
| Density | 1.04 g/mL at 20 °C |
| Melting point | 41-44 °C(lit.) |
| Product parameters | |
| Cas number: | 3483-12-3 |
| Appearance: | White Powder |
| Purity: | 99% min |
| MOQ: | 1g |
| Price: | Negotiable |
| Package details: | 1g/bottle |
| Brand: | Fortuna |
DL-Dithiothreitol (DTT) is a small-molecule reducing agent widely used in biochemistry and molecular biology. Its key features and uses include:
Chemical Structure: A four-carbon sugar derivative with two thiol (-SH) groups (HOCH₂CH(SH)CH(SH)CH₂OH). The "DL" prefix indicates it's a racemic mixture of the D- and L-stereoisomers.
Primary Function:
Reduces disulfide bonds (-S-S-) in proteins and peptides to sulfhydryl groups (-SH + HS-), disrupting protein structure/function.
Prevents/Reverses oxidation of thiol groups, protecting sensitive molecules.
Key Applications:
Protein Denaturation/Unfolding: Essential for SDS-PAGE sample prep.
Enzyme Activity Studies: Maintains cysteine-dependent enzymes in reduced states.
Nucleic Acid Research: Stabilizes ribonucleases, prevents RNA degradation.
Buffer Additive: Prevents unwanted disulfide bond formation (e.g., in chromatography).
Properties:
Strong Reducing Power: Low redox potential.
Water-Soluble: Easily dissolves in aqueous buffers.
Odor: Characteristic sulfurous (rotten egg) smell.
Stability: Air-sensitive (oxidizes to a cyclic disulfide); solutions require preparation fresh or storage under inert gas/freezing.
Safety: Irritant to skin/eyes; handle with gloves in a fume hood.
In short: DTT is an indispensable lab reagent for manipulating disulfide bonds and maintaining reducing environments in biological systems.
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