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5-Bromo-4-chloro-3-indolyl-beta-D-glucoside is used as a biochemical reagent.
5-Bromo-4-chloro-3-indolyl-β-D-glucoside (X-Gluc) is a chromogenic compound used to detect β-glucosidase or β-glucuronidase (GUS) enzyme activity. When the enzyme cleaves X-Gluc, it releases an insoluble blue dye (5,5′-dibromo-4,4′-dichloro-indigo), producing a visible color change. It is widely used in molecular biology as a reporter substrate, especially in genetically modified plants where the gusA gene serves as a marker—blue staining indicates active gene expression. X-Gluc enables precise spatial localization of enzyme activity in plant tissues, seeds, roots, or microbial colonies. It’s also employed in microbiology to screen β-glucosidase-producing bacteria and fungi. Due to its high sensitivity, stability, and clear visual output, X-Gluc is a key tool in genetic engineering, plant research, and enzymatic assays, offering a simple yet powerful method for monitoring gene expression and enzyme function in both research and biotechnology applications.
| Item | Specification |
| Appearance | White Crystalline Powder |
| Assay | 99%min |
| Density | 1.882±0.06 g/cm3(Predicted) |
| Melting point | 249-251 °C |
| Product parameters | |
| Cas number: | 15548-60-4 |
| Appearance: | White Crystalline Powder |
| Purity: | 99% min |
| MOQ: | 1kg |
| Price: | Negotiable |
| Package details: | 1kg/foil bag |
| Brand: | Fortuna |
Abbreviation: X-Gluc
Molecular Formula: C₁₄H₁₆BrClNO₇
Appearance: White to off-white powder
Mechanism: When cleaved by β-glucosidase (or the related enzyme β-glucuronidase, depending on derivative), it releases an insoluble blue indigo dye (5,5′-dibromo-4,4′-dichloro-indigo).
Note: While X-Gluc typically refers to the β-D-glucopyranoside, a closely related compound—X-GlucA (5-bromo-4-chloro-3-indolyl-β-D-glucuronide)—is more commonly used for GUS (β-glucuronidase) assays in plant molecular biology.
Reporter Gene Assays
In genetically modified plants, the gusA gene (encoding GUS enzyme) is fused to a promoter of interest. Adding X-Gluc (or X-GlucA) produces a blue precipitate where the gene is active, enabling visual localization of gene expression.
Enzyme Activity Detection
Used in microbiology and enzymology to screen or quantify β-glucosidase-producing microorganisms (e.g., in soil bacteria or fungi).
Histochemical Staining
Allows spatial mapping of enzyme activity in tissues, roots, seeds, or microbial colonies.
Quality Control in Biotechnology
Validates successful transformation or recombinant protein expression in research and industrial labs.
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